Multiplex bead assay technology relies on the ability of flow cytometers to separate beads covalently coupled to antibodies based on their size and fluorescence intensity. Multiplex bead assays, such as those offered by Assay Designs, can simultaneously quantify up to 36 analytes in a single well by utilizing multiple bead sizes each with different fluorescence intensities. Each bead size is sub-fractioned into different populations based on their inherent fluorescence intensity by a 670 nm filter, while the analytes of interest are simultaneously quantified by a 576 nm filter which measures fluorescence associated with the PE conjugated detection antibody or analyte.
Replacement of traditional ELISA with multiplex immunoassays allows for simultaneous evaluation of multiple analytes using minimal sample volume and offers cost-effective and convenient quantification of biological markers. Best of all, multiplex assaysdo not require special instrumentation and work on the convenient, affordable, dual-laser Accuri C6.
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